![]() ![]() We demonstrate that GAPCps are important for the synthesis of serine in roots. In spite of their low gene expression level as compared with other GAPDHs, GAPCp down-regulation leads to altered gene expression and to drastic changes in the sugar and amino acid balance of the plant. gapcp double mutants display a drastic phenotype of arrested root development, dwarfism, and sterility. In this work, we have identified two Arabidopsis ( Arabidopsis thaliana) chloroplast/plastid-localized GAPDH isoforms (GAPCp1 and GAPCp2). ![]() However, the in vivo functions of the plastidial isoforms remain unresolved. Both cytosolic (GAPCs) and plastidial (GAPCps) GAPDH activities have been described. The glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate with concomitant reduction of NAD + to NADH. ![]() Glycolysis is a central metabolic pathway that, in plants, occurs in both the cytosol and the plastids. ![]()
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